Product Focus 

CLEAN WESTERN BLOTS MADE EASY!

The ReliaBLOT^® IP/Western Blot reagents and procedures (patent pending) provide an improved method for the detection of immunoprecipitated proteins assayed via Western Blot. It seamlessly integrates into existing IP/WB protocols and is cited in peer-reviewed journal articles.

Developmental Regulation of Eed Complex Composition Governs a Switch in Global Histone Modification in Brain
Se Young Kim, et al., J. Biol. Chem., Mar 2007; 282: 9962 - 9972.
...20 mm Tris, pH 7.6, 137 mm NaCl, 0.1% Tween 20) or ReliaBLOT reagent (Bethyl Laboratories) for 2 h at room temperature and incubated...raised against residues 123-140, custom-generated by Bethyl Laboratories, and rabbit polyclonal... 
 
Cytosolic signaling protein Ecsit also localizes to mitochondria where it interacts with chaperone NDUFAF1 and functions in complex I assembly
Rutger O. Vogel, Rolf J.R.J. Janssen, Mariël A.M. van den Brand, Cindy E.J. Dieteren, et al., Genes & Dev., Mar 2007; 21: 615 - 624.
...peroxidase-conjugated anti-mouse or anti-rabbit IgGs. For the detection of immunoprecipitated proteins, ReliaBLOT HRP Conjugate was used (Bethyl Laboratories). The signal was generated using ECL plus...
 
ReliaBLOT is a registered trademark of Bethyl Laboratories, Inc.

NEW POLYCLONAL ANTIBODIES

Each month, antibodies to new target are added to our offering. Here is a sample of what was made available last month.

New Application

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A. Detection of Mouse CBX3 by IHC. 
Sample: FFPE section of mouse teratoma.  Antibody: Affinity purified rabbit anti-CBX3 (Cat. No. IHC-00204) used at a dilution of 1:250.  Detection: DAB staining using Immunohistochemistry Accessory Kit (Cat. No. IHC-101).

B. Detection of Mouse Nanog by IHC. 
Sample: FFPE section of mouse teratoma.  Antibody: Affinity purified rabbit anti-Nanog (Cat. No. IHC-00205) used at a dilution of 1:250.  Detection: DAB staining using Immunohistochemistry Accessory Kit (Cat. No. IHC-101).

C. Detection of Human Reticulocalbin by IHC.  Sample: FFPE section of human breast carcinoma.  Antibody: Affinity purified rabbit anti-Reticulocalbin (Cat. No. IHC-00207) used at a dilution of 1:250.  Detection: DAB staining using Immunohistochemistry Accessory Kit (Cat. No. IHC-101).

D. Detection of Human SUPT5H by IHC. 
Sample: FFPE section of human breast adenocarcinoma.  Antibody: Affinity purified rabbit anti-SUPT5H (Cat. No. IHC-00210) used at a dilution of 1:250.  Detection: DAB staining using Immunohistochemistry Accessory Kit (Cat. No. IHC-101).

RECENT CITATIONS ...

Estrogen Induces Expression of BCAS3, a Novel Estrogen Receptor- Coactivator, through Proline-, Glutamic Acid-, and Leucine-Rich Protein-1 (PELP1)
Anupama E. Gururaj, Shaohua Peng, Ratna K. Vadlamudi, and Rakesh Kumar
Mol. Endocrinol., Aug 2007; 21: 1847 - 1860.
...BCAS3 (Bethyl Laboratories, Montgomery, TX) or PELP1 (Bethyl Laboratories) were used to immunoprecipitate...to Western blot analysis using T7 antibody (Bethyl Laboratories). Cell Extracts, Immunoprecipitation... 

Rapamycin Limits Formation of Active Eukaryotic Initiation Factor 4F Complex Following Meal Feeding in Rat Hearts
Thomas C. Vary, Gina Deiter, and Christopher J. Lynch
J. Nutr., Aug 2007; 137: 1857 - 1862.
...homogenate supernatants using an anti-human RAPTOR antibody (Bethyl Laboratories). RAPTOR and mTOR proteins in the antibody-antigen...anti-human RAPTOR antibody or a rabbit anti-rat mTOR antibody (Bethyl Laboratories). The blots were quantitated...

Arsenite Slows S Phase Progression via Inhibition of cdc25A Dual Specificity Phosphatase Gene Transcription
Geniece M. Lehmann and Michael J. McCabe, Jr
Toxicol. Sci., Sep 2007; 99: 70 - 78.
...min. Lysates were cleared twice by centrifugation. Five hundred microgram protein was incubated with 5 mug rabbit anti-cdc25A (Bethyl Laboratories, Montgomery, TX #A300-076A) antibody 2 h at 4C with end-over-end agitation. Protein-antibody complexes were added...

Detection of Human SAFB2 by WB and IP.  Samples:  Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T: 50 mcg) cells.  Antibodies:  Affinity purified rabbit anti-SAFB2 antibody A301-112A used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.  SAFB2 was also immunoprecipitated by rabbit anti-SAFB2 antibodies A301-113A and BL4609, which recognize downstream epitopes.  For blotting immunoprecipitated SAFB2, ReliaBLOT® Reagents and Procedures (Cat. No. WB120) were used.  Detection:  Chemiluminescence with exposure times of 3 seconds (A and B).