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Detection of Human
Notch1 by WB and IP.
Samples:
Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of
IP loaded) and 293T (T; 50 mcg) cells. Antibodies:
Affinity purified rabbit anti-Notch1 antibody A301-895A
used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg
lysate. Notch1 was also immunoprecipitated by rabbit anti-Notch1 antibody A301-894A,
which recognizes an upstream epitope. For detecting
immunoprecipitated Notch1, ReliaBLOT Reagents and Procedures (Cat. No. WB120)
were used. Detection:
Chemiluminescence with exposure times of 30 seconds (A and B).
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Detection of Human and Mouse HDAC2 by WB (h&m) and IP (h).
Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for
WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M;
50 mcg) cells. Antibodies: Affinity purified rabbit
anti-HDAC2 antibody A300-705A
used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg
lysate. HDAC2 was also immunoprecipitated by rabbit anti-HDAC2 antibody A300-704A,
at 3 mcg/ml lysate. For detecting immunoprecipitated HDAC2, ReliaBLOT
Reagents and Procedures (Cat. No. WB120)
were used. Detection: Chemiluminescence with
exposure times of 30 seconds (A) and 3 seconds (B).
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