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Bethyl Laboratories, Inc.

ReliaBLOT® IP/Western Blot Accessory Kit

ReliaBLOT® IP/Western Blot reagents and procedures provide an improved method for the detection of immunoprecipitated proteins assayed via Western Blot.

Seemless integration into existing IP/WB Procedures with
THREE SIMPLE CHANGES



ReliaBLOT is a registered trademark of Bethyl Laboratories, Inc.

 

Cat. #

 Size

WB120

20 miniblots

WB120T

5 miniblots

 

 ü

Dramatically reduces the usual noise of heavy chain observed when Western Blotting of IP

 ü

Low levels traditionally obscured by the  Ig heavy chain can now be observed

 ü

No changes to your existing optimized IP protocol

 ü

Works well when using Protein-A or Protein-G for IP

 ü

No crosslinking required

 ü

For use when rabbit antibodies are used in Western Blot after IP





Recent Citations

Distinct Protein Arginine Methyltransferases Promote ATP-Dependent Chromatin Remodeling Function at Different Stages of Skeletal Muscle Differentiation.
Caroline S. Dacwag, Mark T. Bedford, Saïd Sif, and Anthony N. Imbalzano
Mol. Cell. Biol., Apr 2009; 29: 1909 - 1921.
...centrifugation to obtain a cell pellet. Carm1 Western analysis was performed by using cell lysates from immortalized MEFs using the ReliaBlot protocol (Bethyl Laboratories), while all other cell pellets were resuspended in NP-40 lysis buffer...
 
 
Inhibition of the KCa3.1 channels by AMP-activated protein kinase in human airway epithelial cells.
Hélène Klein, Line Garneau, Nguyen Thu Ngan Trinh, Anik Privé, François Dionne, Eugénie Goupil, Domi nique Thuringer, Lucie Parent, Emmanuelle Brochiero, and Rémy Sauvé
Am J Physiol Cell Physiol, Feb 2009; 296: C285 - C295.

...respectively, was eliminated by blocking membranes with IP/Western blot ReliaBLOT reagents (Bethyl Laboratories, Montgomery, TX) overnight. The membranes were then incubated in ReliaBLOT with either rabbit anti-AMPK-1 antibody... 
 
The tumor suppressor Cdc73 functionally associates with CPSF and CstF 3' mRNA processing factors.
Orit Rozenblatt-Rosen, Takashi Nagaike, Joshua M. Francis, Syuzo Kaneko, Karen A. Glatt, Christina M. Hughes, Thomas LaFramboise, James L. Manley, and Matthew Meyerson
PNAS, Jan 2009; 106: 755 - 760.
...Antibodies were diluted in 5% milk/ TBST according to the manufacturer's instructions. A ReliaBlot kit (Bethyl Laboratories) was used to avoid masking of protein bands by the Ig heavy chain...

Search for Antibodies by Research Area
Bethyl Laboratories' portfolio of polyclonal antibodies commercially available includes over 3500 polyclonal antibodies to over 1300 proteins. We test our antibodies in a wide range of applications including ELISA, western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry. We have recently validated many of our antibodies for flow cytometry and ChIP. Our antibody range also includes secondary antibodies, loading controls and antibodies to epitope tags.

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