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New Products from Enzo Life Sciences
43/2009
View all the New Products on our Website  NEW Antibodies Section
LSD1 Fluorometric Drug Discovery Kit
A single-step, homogenous assay for LDS1 inhibitor screening. LSD1 (KDM1; lysine-specific histone demethylase 1; AOF2), a flavin-containing amine oxidase homolog and component of various corepressor complexes, catalyzes the oxidative demethylation of mono- and demethylated lysines in histones, p53 and DNMT1. There is increasing evidence for LSD1 playing epigenetic and transcriptional roles in processes ranging from embryogenesis to carcinogenesis. The CELLestialTM Red Substrate detection system allows sensitive realtime fluorometric or colorimetric detection of LSD1 activity. Over 1000U of LSD1 are supplied with each kit.
Mito-ID™ Membrane Potential Detection Kit for microscopy and flow cytometry
Highly sensitive assays for monitoring decrease in mitochondrial membrane potential. Mito-ID™ dye detects membrane potential loss at one-tenth the drug/test agent concentration required with other commonly used dyes, such as JC-1. Loss of orange signal, localized to energized mitochondria, indicates a decline in mitochondrial membrane potential. Kit also available for microplates.
Figure: The Orange fluorescent aggregates are localized in the mitochondria (orange channel), while green fluorescent monomers mainly stain the cytosol (FITC channel).
Related Product: Mito-ID™ Membrane Potential Cytotoxicity Kit for microplates
SDMA (human) ELISA Kit
For the quantitative determination of SDMA (symmetrical dimethylarginine) in human EDTA-plasma and serum. Does not cross-react with ADMA, NMMA or L-arginine. SDMA is a methylated derivative of L-Arginine which is strictly eliminated by renal extraction. It exhibits properties of a reliable marker of renal dysfunction as SDMA plasma level is strongly correlated to renal function.
Related Products: ADMA (human) ELISA Kit / NG, N'G-Dimethyl-L-arginine . 2 HABS / NG, N'G-Dimethyl-L-arginine . 2 HCl
New Probes for Enzymes with Deubiquitinylating Activity:
Ubiquitin Vinyl Sulfone (HA-tagged)
Ubiquitin Vinyl Methyl Ester (HA-tagged)
Chloroethyl Ubiquitin (HA-tagged)
Bromoethyl Ubiquitin (HA-tagged)
Hemagglutinin (HA)-tagged ubiquitin molecules derivatized at the C-terminus with thiol-reactive functional groups can be used as potent, irreversible inhibitors of deubiquitinylating enzymes (DUBs). They can act as specific probes for enzymes with DUB activity through their covalent modification of the enzyme active site.
LIT: A. Borodovsky, et al.; EMBO J. 20, 5187 (2001) • A. Borodovsky, et al.; Chem. Biol. 9, 1149 (2002) • H. Ovaa, et al.; PNAS 101, 2253 (2004) • M. J. Edelmann, et al.; Biochem. J. 418, 379 (2009)
Related Products: Browse all ubiquitin and ubl deconjugating enzymes
BACE1 (human) (rec.) - An aspartic protease involved in Alzheimer's disease and cancer
Active soluble BACE1 produced in E. coli.
Related Products: PAb to BACE1 / PAb to BACE2
DESC1 (human) (rec.) - A trypsin-like serine protease
Type II transmembrane serine protease which belongs to an emerging class of proteolytic enzymes involved in tissue homeostasis and a number of human disorders such as cancer.
LIT: F. Beliveau, et al.; FEBS J. 276, 2213 (2009)
Related Products: Matriptase (human) (rec.) / Matriptase-2 (human) (rec.) / Hepsin (human) (rec.)
(S)-(+)-Ascochin - Isocoumarin derivative with antifungal and phytotoxic properties
Quenched fluorogenic substrate for ADAM8, -10, -12, -17 (TACE) and likely other ADAMs. Also efficiently cleaved by MMP-1, -2, -3, -8, -9, -13, and -14.
LIT: K. Krohn, et al.; Eur. J. Org. Chem. 7, 1123 (2007)
Niphimycin - An antifungal antibiotic
The fungicidal activity appears to result from membrane damage and enhanced oxidative stress.
LIT: L. Bassi, et al.; Helv. Chim. Acta 66, 92 (1983) • P. Gassman, et al.; Helv. Chim. Acta 67, 696 (1984) • K. Nakayama, et al.; J. Biosci. Bioeng. 94, 207 (2002)
Related Product: 19-Malonyl-niphimycin
HC-030031 - A potent and selective TRPA1 inhibitor
LIT: C. R. McNamara, et al.; PNAS 104, 13525 (2007)
Related Products: TRPA ligands & antibodies / All TRP channel products
AP-18 - A TRPA1 blocker
Inhibits TRPA1 (CHO cells, IC50= 3.1 µM; human and mouse cell lines, IC50= 4.5 µM). AP-18 was unable to block activation of TRPV1, TRPV2, TRPV3, TRPV4 or TRPM8 at concentrations up to 50μM.
LIT: M. Petrus, et al.; Mol. Pain. 3, 40 (2007)
Related Products: TRPA ligands & antibodies / All TRP channel products
N-Arachidonoyl-serotonin - A TRPV1 antagonist
Unnatural fatty acid-neurotransmitter conjugate. Potent TRPV1 antagonist (IC50=40nM). Inhibits fatty acid amide hydrolase (FAAH) (IC50=5.6mM).
LIT: S. Maione, et al.; Br. J. Pharmacol. 150, 766 (2007)
Related Products: TRPA ligands & antibodies / All TRP channel products
ENMD-1068 - Selective proteinase-activated receptor-2 (PAR-2) antagonist
Dose dependently inhibits PAR-2 but not PAR-3 or PAR-4 signaling. Reduces TNF-α and IL-1β production from rheumatoid arthritis synovial primary cell culture and reduces joint swelling in a carageenan/kaolin-injected mouse model.
LIT: E. B. Kelso, et al.; J. Pharmacol. Exp. Ther. 316, 1017 (2006) • E. B. Kelso, et al.; Arthritis Rheum. 56, 765 (2007)
Related Products: PAb to PAR-1 (Extracellular) / PAb to PAR-2 / PAb to PAR-4 |
